詳解小鼠靜脈注射操作步驟
<p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">上海酶聯(lián)生物科技有限公司</span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">的小鼠靜脈注射這也是常見的操作,稍微有點難度,沒有指導(dǎo)的話,一開始可能會感覺有點手足無措。但是可以肯定的說,只要掌握了方法,小鼠的尾靜脈注射還是很容易的。</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="background-color: rgb(255, 255, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">操作步驟:</span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">1. </span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">首先要固定小鼠,簡單的固定方法就是把小鼠放在盒子里面,讓它的尾巴伸在盒蓋的外面,用手抓住小鼠尾巴,輕輕往外拽,就可以固定好小鼠了。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">這種固定方法,小鼠可以在盒子里面活動,固定的也不是很牢固,但是只要你尾靜脈注射的手法很熟練,就足以用來注射了。</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">還有的固定方法就是用一個小的圓筒,是金屬做的,(可以在當(dāng)?shù)氐蔫F匠鋪,或者買白鐵鋪里面定做)首先是金屬比較結(jié)實,而且可以用來固定在鐵架臺上,方便操作。圓筒的一段有個蓋子可以拿下來,蓋子中間有個小孔,可以讓小鼠的尾巴伸出來(中間的小孔可以用膠布纏一下,防止銳利的邊緣割傷小鼠尾巴)。</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">另外一段可以用金屬網(wǎng)的結(jié)構(gòu),網(wǎng)的形狀可以做成頭的頭端形狀。網(wǎng)狀結(jié)構(gòu)可以讓光線透近來,方便小鼠鉆進(jìn)圓筒里面。圓筒的長度約</font>10cm<font face="宋體">,直徑約 </font><font face="Calibri">3</font><font face="宋體">~</font><font face="Calibri">4cm</font><font face="宋體">,可以做個系列長度和直徑的圓筒,適合不同大小的小鼠。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">2. </span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">固定好小鼠后就是注射了,一般用一次性的</font><font face="Calibri">1ml</font><font face="宋體">的注射器就可以了,玻璃 的</font><font face="Calibri">1ml</font><font face="宋體">的注射器也可以用,針頭用</font><font face="Calibri">4</font><font face="宋體">號的就可以了。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">3. </span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">注射前首先要讓小鼠的血管充盈??梢圆捎?lt;/font><font face="Calibri">75%</font><font face="宋體">的酒精棉球擦拭的方法或者采用溫水浸泡的方法。若小鼠的血管很不清楚,推薦采用溫水浸泡的方法,水溫以不燙手為宜。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">溫水浸泡</font>2<font face="宋體">~</font><font face="Calibri">3</font><font face="宋體">分鐘后,取出小鼠尾巴,用干棉球擦拭。等一會兒,待血管充盈后,酒精棉球擦拭后就可以進(jìn)針了。若血管還不充盈,可以反復(fù)用溫水浸泡,切不可冒險注射,除非你手法很熟練,另當(dāng)別論。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">4. </span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">小鼠尾部共有四條血管,一般認(rèn)為左右的兩條靜脈比較容易注射,多采用這兩條靜脈進(jìn)針。一般要求進(jìn)針部位靠近小鼠的尾端,這樣若注射失敗的話,還可以再向上選擇進(jìn)針點。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">但是進(jìn)針部位也不可以太靠下,因為越往下,靜脈越細(xì),操作越難,一般以小鼠尾巴下三分的位置比較好。</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">5.</span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> <font face="宋體">關(guān)鍵的就是進(jìn)針了。進(jìn)針時操作者左手食指和拇指固定住小鼠的尾巴,讓小鼠的尾巴在經(jīng)過拇指后向下彎曲,進(jìn)針點靠近拇指指甲。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">針頭和血管呈約</font>30<font face="宋體">°角,針尖斜面朝上,輕輕挑刺入皮膚后針頭立即和血管平行,一般情況下一次就可以進(jìn)入血管,可以將針頭刺入血管一大半,左右輕輕晃動針頭,確定針頭在血管內(nèi),就可以推注藥液了,正常情況下,推注的過程應(yīng)該沒有明顯阻力,血管也不會鼓起。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">推液時動作宜輕柔,若發(fā)現(xiàn)血管鼓起,那是針頭沒有刺入血管,需立即拔出針頭。</span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>