酶聯(lián)生物抗體的選擇對策
<p class="MsoNormal"><strong><span style="color: rgb(255, 102, 0);"><span style="font-family: 宋體; font-size: 9pt;">★ </span><span style="color: rgb(0, 0, 0);"><span style="font-family: 宋體; font-size: 9pt;">分析您的試驗應(yīng)用的類型 </span></span></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">一般抗體說明書都列出該抗體經(jīng)試驗驗證過適用于何種分析類型,如:可以應(yīng)用于</font>WB IHC ICC ELASA<font face="宋體">分析等,如果抗體說明書沒有提及的應(yīng)用類型。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">并不意味著該抗體不適用于此種分析應(yīng)用類型,而僅是說明尚未經(jīng)過此種分析試驗驗證,如果抗體不適用某些分析試驗,則會在抗體說明書上標(biāo)注出來不適于某分析試驗。</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><strong><span style="color: rgb(255, 0, 0);"><span style="font-family: 宋體; font-size: 9pt;">★</span><span style="color: rgb(0, 0, 0);"><span style="font-family: 宋體; font-size: 9pt;"> 樣本的物種</span></span></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">應(yīng)選擇物種相同或有交叉反應(yīng)的抗體,抗體可能與不同物種的同種靶蛋白有交叉反應(yīng),因其氨基酸序列同源性較高,如果樣本的種類未列入抗體說明書上的交叉反應(yīng)種屬表中。</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">并不意味著該抗體不適用于檢測該物種的蛋白,而只是表示該物種尚未用此抗體檢測驗證過,應(yīng)通過序列比對的方法來預(yù)測交叉反應(yīng),可應(yīng)用</font>Expasy <font face="宋體">和 </font><font face="Calibri">NCBI BLAST</font><font face="宋體">來進(jìn)行不同物種蛋白同源性比對。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><strong><span style="color: rgb(255, 102, 0);"><span style="font-family: 宋體; font-size: 9pt;">★ </span><span style="color: rgb(0, 0, 0);"><span style="font-family: 宋體; font-size: 9pt;">雙重染色抗體的選擇</span></span></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">用未偶聯(lián)一抗進(jìn)行細(xì)胞培養(yǎng)物或組織切片的雙重免疫染色要求一抗來源于不同物種并且二抗分別識別其中之一,二抗說明書應(yīng)描述其與其它物種來源的免疫球蛋有否有交叉吸附。</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>