白介素類(lèi)的elisa試劑盒實(shí)驗(yàn)條件
<p class="MsoNormal"><span style="background-color: rgb(0, 255, 255);"><strong><span style="font-family: 宋體; font-size: 9pt;">實(shí)驗(yàn)條件</span></strong></span><span style="font-family: 宋體; font-size: 9pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><strong><span style="font-family: 宋體; font-size: 9pt;">elisa<font face="宋體">試劑盒</font></span></strong><span style="font-family: 宋體; font-size: 9pt;"><font face="宋體">白介素類(lèi)檢測(cè)抗體多用間接型</font><font face="Calibri">elisa</font><font face="宋體">,以純化抗原包被載體,經(jīng)</font><font face="Calibri">4</font><font face="宋體">℃過(guò)夜,以無(wú)關(guān)蛋白封閉至少</font><font face="Calibri">2</font><font face="宋體">小時(shí)以上,加入待測(cè)樣品,室溫孵育</font><font face="Calibri">1</font><font face="宋體">小時(shí),洗滌干凈后加酶標(biāo)抗體,再經(jīng)室溫孵育、洗滌后,以底物顯色,顯色到一定程度終止反應(yīng),在酶標(biāo)儀上讀結(jié)果或以肉眼判斷。</font></span><span style="font-family: 宋體; font-size: 9pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">固相載體:</span></strong></span><span style="font-family: 宋體; font-size: 9pt;"><o:p></o:p></span></p>
<p><font face="宋體" style="font-family: 宋體; font-size: 9pt;">可作為固相載體的物質(zhì)很多,如:纖維素、聚丙烯酰胺、聚苯乙烯、聚丙烯、交聯(lián)葡聚糖、玻璃、硅橡膠、瓊脂糖凝膠等。可以各種形式出現(xiàn);試管、微量反應(yīng)板凹孔、小珠</font><span style="font-family: 宋體; font-size: 9pt;">.....</span><font face="宋體" style="font-family: 宋體; font-size: 9pt;">常用的是聚苯乙烯制品,其吸附性</font><font face="宋體" style="font-family: 宋體; font-size: 9pt;">好,固相包被的機(jī)制一般不外乎物理吸附和共價(jià)交聯(lián),</font><font face="宋體" style="font-family: 宋體; font-size: 9pt;">類(lèi)</font><span style="font-family: 宋體; font-size: 9pt;"> </span><font face="宋體" style="font-family: 宋體; font-size: 9pt;">型:</font></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">elisa<font face="宋體">具體方法的類(lèi)型繁多,試劑的標(biāo)準(zhǔn)化問(wèn)題還未</font><font face="Calibri">zui</font><font face="宋體">后解決,溫育的時(shí)間、溫度、洗滌液的種類(lèi)及洗滌次數(shù)等都是影響測(cè)定的因素。但參考以上基本過(guò)程,</font></span><strong><span style="font-family: 宋體; font-size: 9pt;">elisa<font face="宋體">試劑盒</font></span></strong><font face="宋體" style="font-family: 宋體; font-size: 9pt;">無(wú)論怎樣變化,均離不開(kāi)抗原</font><font face="Calibri" style="font-size: 9pt;">-</font><font face="宋體" style="font-family: 宋體; font-size: 9pt;">抗體反應(yīng)、酶標(biāo)抗體的免疫學(xué)反應(yīng)及酶催化作用。</font></p>
<p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;"><font face="宋體">特</font> <font face="宋體">點(diǎn):</font></span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1<font face="宋體">、敏感性高,可檢出濃度在</font><font face="Calibri">ug~mg/ml</font><font face="宋體">水平;</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2<font face="宋體">、特異性強(qiáng);</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">3<font face="宋體">、重復(fù)性好;</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">4<font face="宋體">、快速、簡(jiǎn)便、廉價(jià)。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">封閉:</span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">以無(wú)關(guān)蛋白占據(jù)載體上未被包被的空隙,常用的封閉液是</font>1%<font face="宋體">牛血清白蛋白或</font><font face="Calibri">10%</font><font face="宋體">小牛血清。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">待測(cè)樣品:</span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">稀釋度要合適,濃度太大會(huì)發(fā)生非特異性吸附造成假陽(yáng)性,過(guò)分稀釋會(huì)影響抗原</font>-<font face="宋體">抗體之間的免疫學(xué)反應(yīng)。選擇</font><font face="Calibri">zui</font><font face="宋體">適稀釋度,在實(shí)驗(yàn)體系中取光密度在</font><font face="Calibri">1.0</font><font face="宋體">左右的稀釋度為宜。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="color: rgb(255, 102, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">酶結(jié)合物:</span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><strong><span style="font-family: 宋體; font-size: 9pt;">elisa<font face="宋體">試劑盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">使用高質(zhì)量的酶結(jié)合物是</font>elisa<font face="宋體">成功的關(guān)鍵。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>