elisa試劑盒的檢測(cè)前準(zhǔn)備工作及注意事項(xiàng)

<p class="MsoNormal"><strong><span style="color: rgb(255, 0, 0);"><span style="font-family: 宋體; font-size: 9pt;">檢測(cè)前準(zhǔn)備工作：</span></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1.</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> <font face="宋體">請(qǐng)?zhí)崆?lt;/font><font face="Calibri">20</font><font face="宋體">分鐘從冰箱中取出試劑盒，以平衡至室溫。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2. </span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">將濃縮洗滌液用雙蒸水稀釋（</font><font face="Calibri">1:20</font><font face="宋體">）。未用完的放回</font><font face="Calibri">4</font><font face="宋體">℃。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">3.</span></strong><span style="font-size: 9pt;"> <font face="宋體" style="font-family: 宋體;">標(biāo)準(zhǔn)品</font></span><span style="font-family: 宋體;">：</span><span style="font-size: 9pt;"><font face="宋體" style="font-family: 宋體;">加入標(biāo)準(zhǔn)品</font><font face="Calibri" style="font-family: 宋體;">/</font><font face="宋體" style="font-family: 宋體;">標(biāo)本稀釋液</font><font face="Calibri" style="font-family: 宋體;">0.5ml</font><font face="宋體" style="font-family: 宋體;">至凍干標(biāo)準(zhǔn)品中，靜置</font><font face="Calibri" style="font-family: 宋體;">15</font><font face="宋體" style="font-family: 宋體;">分鐘，待其充分溶解后，輕輕混勻（濃度為</font><font face="Calibri" style="font-family: 宋體;">1000 pg/ml</font><font face="宋體" style="font-family: 宋體;">）。然后根據(jù)需要進(jìn)行稀釋。（建議標(biāo)準(zhǔn)曲線使用以下濃度： </font><font face="Calibri" style="font-family: 宋體;">1000</font><font face="宋體" style="font-family: 宋體;">、</font><font face="Calibri" style="font-family: 宋體;">500</font><font face="宋體" style="font-family: 宋體;">、</font><font face="Calibri" style="font-family: 宋體;">250</font><font face="宋體" style="font-family: 宋體;">、</font><font face="Calibri" style="font-family: 宋體;">125</font><font face="宋體" style="font-family: 宋體;">、</font><font face="Calibri" style="font-family: 宋體;">62.5</font><font face="宋體" style="font-family: 宋體;">、</font><font face="Calibri" style="font-family: 宋體;">31.25</font><font face="宋體" style="font-family: 宋體;">、</font><font face="Calibri" style="font-family: 宋體;">15.6</font><font face="宋體" style="font-family: 宋體;">、</font><font face="Calibri" style="font-family: 宋體;">0 pg/ml</font><font face="宋體" style="font-family: 宋體;">）。復(fù)溶標(biāo)準(zhǔn)品原液（</font><font face="Calibri" style="font-family: 宋體;">1000 pg/ml</font><font face="宋體" style="font-family: 宋體;">）若未用完請(qǐng)分裝后放入</font><font face="Calibri" style="font-family: 宋體;">-20</font><font face="宋體" style="font-family: 宋體;">℃以下電冰箱內(nèi)保存，保存兩個(gè)月。已稀釋的標(biāo)準(zhǔn)品請(qǐng)廢棄。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">4.</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> <font face="宋體">生物素化抗體工作液：按當(dāng)次試驗(yàn)所需要得用量，使用前</font><font face="Calibri">20</font><font face="宋體">分鐘，以生物素化抗體稀釋液稀釋濃縮生物素化抗體（</font><font face="Calibri">1:30</font><font face="宋體">）成工作濃度。當(dāng)日使用。若實(shí)驗(yàn)次數(shù)過(guò)多導(dǎo)致生物素化 抗體量不足，可向我們公司單獨(dú)購(gòu)買生物素化抗體。（須提供抗體批號(hào)）</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">5.</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"> <font face="宋體">酶結(jié)合物工作液：按當(dāng)次試驗(yàn)所需要得用量，使用前</font><font face="Calibri">20</font><font face="宋體">分鐘，以酶結(jié)合物稀釋液稀釋濃 縮酶結(jié)合物（</font><font face="Calibri">1:30</font><font face="宋體">） 成工作濃度。當(dāng)日使用。若實(shí)驗(yàn)次數(shù)過(guò)多導(dǎo)致濃縮酶結(jié)合物量不足， 可向我們公司單獨(dú)購(gòu)買濃縮酶結(jié)合物。（須提供酶結(jié)合物批號(hào)）</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">洗滌方法：</span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1. <font face="宋體">自動(dòng)洗板機(jī)：要求注入的洗滌液為</font><font face="Calibri">350ul</font><font face="宋體">，注入與吸出間隔</font><font face="Calibri">15</font><font face="宋體">－</font><font face="Calibri">30</font><font face="宋體">秒。洗板</font><font face="Calibri">5</font><font face="宋體">次。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2. <font face="宋體">手工洗板：甩盡孔內(nèi)液體，在潔凈的吸水紙上拍干，每孔加洗滌液</font><font face="Calibri">350ul</font><font face="宋體">，靜置</font><font face="Calibri">30</font><font face="宋體">秒后甩盡液體，在厚迭吸水紙上拍干。洗板</font><font face="Calibri">5</font><font face="宋體">次。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="color: rgb(255, 0, 0);"><strong><span style="font-family: 宋體; font-size: 9pt;">注意事項(xiàng)：</span></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">1. <font face="宋體">試劑盒使用前請(qǐng)保存在</font><font face="Calibri">2-8</font><font face="宋體">℃。復(fù)溶后的標(biāo)準(zhǔn)品應(yīng)分裝后，將其放在</font><font face="Calibri">-20</font><font face="宋體">～</font><font face="Calibri">-70</font><font face="宋體">℃貯存。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">2. <font face="宋體">濃縮生物素化抗體，濃縮酶結(jié)合物體積小，運(yùn)輸中顛簸和可能的倒置，會(huì)使液體沾到管壁 或瓶蓋。因此使用前請(qǐng)用手甩幾下或</font><font face="Calibri">1000</font><font face="宋體">轉(zhuǎn)</font><font face="Calibri">/</font><font face="宋體">分離心</font><font face="Calibri">1</font><font face="宋體">分鐘，以使附著管壁或瓶蓋的液體沉積到管底。取用前，請(qǐng)用移液器小心吹打</font><font face="Calibri">4-5</font><font face="宋體">次使溶液混勻。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">3. <font face="宋體">從冰箱中取出的濃縮洗滌液可能有結(jié)晶</font><font face="Calibri">,</font><font face="宋體">屬于正常現(xiàn)象，微加熱至</font><font face="Calibri">40</font><font face="宋體">℃使結(jié)晶完全溶解后 再配制洗滌液。（加熱溫度不要超過(guò)</font><font face="Calibri">50</font><font face="宋體">℃）使用時(shí)洗滌液應(yīng)為室溫。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">4. <font face="宋體">若需要分次使用標(biāo)準(zhǔn)品應(yīng)按照每一次用量分裝，將其放在</font><font face="Calibri">-20</font><font face="宋體">～</font><font face="Calibri">-70</font><font face="宋體">℃貯存，避免反復(fù)凍 融。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">5. <font face="宋體">不同批號(hào)的試劑盒組份不能混用（洗滌液和反應(yīng)終止液除外）。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">6. <font face="宋體">充分輕微混勻?qū)Ψ磻?yīng)結(jié)果尤為重要，使用微量振蕩器（使用</font><font face="Calibri">*</font><font face="宋體">頻率），如無(wú)微量振蕩器，可在反應(yīng)前手工輕輕敲擊酶標(biāo)板框混勻。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">7. <font face="宋體">在試驗(yàn)中標(biāo)準(zhǔn)品和樣本檢測(cè)時(shí)建議作雙孔檢測(cè)。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">8. <font face="宋體">試驗(yàn)中所用的試管和吸頭均為一次性使用，嚴(yán)禁在不同的液體之間混用！否則將影響試 驗(yàn)結(jié)果。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">9. <font face="宋體">試驗(yàn)中請(qǐng)穿著試驗(yàn)服并帶乳膠手套做好防護(hù)工作，特別是檢測(cè)血液或者其他體液標(biāo)本時(shí)， 請(qǐng)按國(guó)家生物試驗(yàn)室安全防護(hù)條列執(zhí)行。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>