什么是ELISA 稀釋劑？一文了解概述、類型和應(yīng)用
<p> <font face="宋體" style="font-family: 宋體; font-size: 10.5pt;"> 酶聯(lián)免疫吸附試驗(yàn)</font><font face="Calibri" style="font-size: 10.5pt;">(ELISA)</font><font face="宋體" style="font-family: 宋體; font-size: 10.5pt;">是一種常用的基于微孔板（例如</font><font face="Calibri" style="font-size: 10.5pt;">96</font><font face="宋體" style="font-family: 宋體; font-size: 10.5pt;">孔板）的檢測(cè)方法，廣泛應(yīng)用于免疫學(xué)和生物化學(xué)實(shí)驗(yàn)室。它利用抗原抗體反應(yīng)的原理，可以定性和定量分析各種生物分子和化學(xué)物質(zhì)，例如肽、蛋白質(zhì)、抗體、激素、小分子、藥物和毒素等。</font></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> </font><font face="Calibri">ELISA</font><font face="宋體">利用一系列試劑來檢測(cè)目標(biāo)物質(zhì)。其中最關(guān)鍵的試劑包括包被抗原</font><font face="Calibri">/</font><font face="宋體">抗體、檢測(cè)抗體、底物和終止液。此外，還需要其他輔助試劑，如封閉液和洗滌液，以確保檢測(cè)的準(zhǔn)確性和靈敏度。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> </font><font face="Calibri">ELISA</font><font face="宋體">中常用的稀釋劑包括樣品稀釋劑和測(cè)定稀釋劑（或抗體稀釋劑）。樣品稀釋劑用于稀釋待測(cè)樣品，而測(cè)定稀釋劑用于稀釋檢測(cè)抗體。兩者都有助于降低背景噪音，提高檢測(cè)的準(zhǔn)確性。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> 稀釋劑如何在測(cè)定中發(fā)揮作用并與其他成分相互作用以提供結(jié)果？哪些工作流程涉及使用稀釋劑？哪些行業(yè)經(jīng)常使用它們？下面由酶聯(lián)生物詳細(xì)為您解答。</span></p>
<p class="MsoNormal"> </p>
<p class="MsoNormal" style="text-align: center;"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><img src="/images/upload/Image/10(23).png" width="500" height="375" alt="酶聯(lián)生物elisa試劑盒" /></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<h3><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> </font><font face="Calibri">ELISA</font><font face="宋體">稀釋劑有哪些作用？</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></h3>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> </font><font face="Calibri">ELISA</font><font face="宋體">實(shí)驗(yàn)中會(huì)用到多種試劑，包括包被緩沖液、封閉緩沖液、結(jié)合物穩(wěn)定劑、底物、終止液以及各種稀釋劑。其中，稀釋劑主要包括樣品稀釋劑和抗體</font><font face="Calibri">/</font><font face="宋體">測(cè)定稀釋劑。樣品稀釋劑用于稀釋待測(cè)樣品，而抗體</font><font face="Calibri">/</font><font face="宋體">測(cè)定稀釋劑用于稀釋檢測(cè)抗體，并提供最佳的反應(yīng)環(huán)境。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> </font><font face="Calibri">ELISA</font><font face="宋體">還需要標(biāo)準(zhǔn)品和待測(cè)樣品。標(biāo)準(zhǔn)品是已知濃度的目標(biāo)分析物，用于繪制標(biāo)準(zhǔn)曲線。待測(cè)樣品則包含未知濃度的目標(biāo)分析物。由于樣品基質(zhì)的復(fù)雜性，可能會(huì)對(duì)檢測(cè)結(jié)果造成干擾。為了減少基質(zhì)效應(yīng)，需要進(jìn)行樣品預(yù)處理或選擇合適的樣品稀釋劑。樣品基質(zhì)和標(biāo)準(zhǔn)品基質(zhì)的差異應(yīng)該通過標(biāo)準(zhǔn)品的選擇和樣品預(yù)處理來盡量減小，而不是依賴于檢測(cè)稀釋劑。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<h3><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> </font><font face="Calibri">ELISA</font><font face="宋體">稀釋劑的類型</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></h3>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> 在</font><font face="Calibri">ELISA</font><font face="宋體">實(shí)驗(yàn)中經(jīng)常用到</font><font face="Calibri">2</font><font face="宋體">種類型稀釋劑，包括樣品稀釋劑和測(cè)定稀釋劑</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> 樣品稀釋液是間接</font><font face="Calibri">ELISA</font><font face="宋體">檢測(cè)的第一步中用于評(píng)估抗體和蛋白質(zhì)的特定緩沖溶液。在免疫檢測(cè)（例如</font><font face="Calibri">ELISA</font><font face="宋體">和</font><font face="Calibri">WesternBlotting</font><font face="宋體">檢測(cè)）中，稀釋液有助于：</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<ol>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;">盡量減少非特異性結(jié)合</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;">降低背景噪音</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;">減少樣本變異</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
</ol>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> 市場(chǎng)上可用的樣品稀釋劑類型包括：</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<ol>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> <strong>血漿樣本稀釋液</strong>：一種富含添加劑的</font><font face="Calibri">BSA</font><font face="宋體">緩沖液，可在</font><font face="Calibri">ELISA</font><font face="宋體">板孵育階段防止凝血（凝血酶抑制）和補(bǔ)體激活。它可用于血漿樣本的抗原向下</font><font face="Calibri">ELISA</font><font face="宋體">格式和血漿樣本的抗體夾心</font><font face="Calibri">ELISA</font><font face="宋體">。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> <strong>通用血清稀釋劑</strong>：緩沖</font><font face="Calibri">BSA</font><font face="宋體">溶液，在特定抗原抗體相互作用測(cè)定過程中起到維持恒定溶質(zhì)和</font><font face="Calibri">pH</font><font face="宋體">環(huán)境的作用。它們經(jīng)常用于稀釋哺乳動(dòng)物和雞血清樣本。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> </font><strong><font face="Calibri">Neptune</font><font face="宋體">樣品稀釋劑</font></strong><font face="宋體">：一種非哺乳動(dòng)物蛋白質(zhì)溶液。它與血清和血漿樣品一起用于抗體夾心</font><font face="Calibri">ELISA</font><font face="宋體">檢測(cè)。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
</ol>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> <strong>樣品稀釋劑優(yōu)化包</strong>：上述三種稀釋劑的混合物。用于確定哪種稀釋劑適合您的研究用途。</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> 抗體</font><font face="Calibri">/</font><font face="宋體">檢測(cè)抗體稀釋劑，主要用于稀釋</font><font face="Calibri">ELISA</font><font face="宋體">中的檢測(cè)抗體，并提供最佳的抗原抗體反應(yīng)環(huán)境。它有助于提高檢測(cè)的靈敏度、特異性和穩(wěn)定性。用途如下：</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<ol>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> </font><strong><font face="宋體">解決抗原向下或抗體夾心</font><font face="Calibri">ELISA</font><font face="宋體">中的基質(zhì)問題</font></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;">減少樣品基質(zhì)干擾</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;">盡量減少非特異性相互作用</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;">降低背景噪音</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
</ol>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> 實(shí)驗(yàn)室應(yīng)用中使用的分析稀釋劑的類型包括：</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<ol>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> <strong>通用檢測(cè)稀釋劑</strong>：抑制凝血和補(bǔ)體活性，廣泛用于夾心</font><font face="Calibri">ELISA</font><font face="宋體">檢測(cè)中的血漿或血清樣本。</font><font face="Calibri"> </font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> <strong>海王星測(cè)定稀釋劑</strong>：抑制凝血酶和補(bǔ)體活性。在抗原下降</font><font face="Calibri">ELISA</font><font face="宋體">中與血清和血漿樣本一起使用。</font><font face="Calibri"> </font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> </font><strong><font face="Calibri">IgM</font><font face="宋體">還原測(cè)定稀釋劑</font></strong><font face="宋體">：通過抑制補(bǔ)體和凝血酶活性，同時(shí)最大限度地減少</font><font face="Calibri">IgM</font><font face="宋體">介導(dǎo)的結(jié)合橋接干擾來發(fā)揮作用。它在夾心</font><font face="Calibri">ELISA</font><font face="宋體">中與血漿和血清樣本一起使用。</font><font face="Calibri"> </font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> <strong>抗原降低測(cè)定稀釋劑</strong>：用于防止</font><font face="Calibri">ELISA</font><font face="宋體">微孔板中樣品凝結(jié)。適用于血漿和血清樣品的抗原降低</font><font face="Calibri">ELISA</font><font face="宋體">。</font><font face="Calibri"> </font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> <strong>分析稀釋劑優(yōu)化包</strong>：包含上述所有四種稀釋劑，幫助您確定哪種稀釋劑最適合您的研究應(yīng)用。</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
</ol>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<h3><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> 哪些行業(yè)使用</font><font face="Calibri">ELISA</font><font face="宋體">稀釋劑？</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></h3>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> </font><font face="Calibri">ELISA</font><font face="宋體">檢測(cè)稀釋劑是一種緩沖溶液（通常是含有牛血清溶液的磷酸鹽緩沖鹽水</font><font face="Calibri">(PBS)</font><font face="宋體">），用于稀釋</font><font face="Calibri">ELISA</font><font face="宋體">樣品或均衡檢測(cè)基質(zhì)。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> 該緩沖液專為</font><font face="Calibri">ELISA</font><font face="宋體">應(yīng)用而設(shè)計(jì)并驗(yàn)證，包括：</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<ol>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> </font><strong><font face="宋體">阻斷抗體包被的</font><font face="Calibri">ELISA</font><font face="宋體">板微孔</font></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> </font><strong><font face="宋體">稀釋</font><font face="Calibri">ELISA</font><font face="宋體">標(biāo)準(zhǔn)品和樣品</font></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></li>
<li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> <strong>稀釋生物素化的檢測(cè)抗體或三級(jí)試劑</strong>（例如</font><font face="Calibri">Avidin-HRP</font><font face="宋體">或</font><font face="Calibri">Streptavidin-HRP</font><font face="宋體">）。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
</ol>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;">藥品</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> </font><font face="Calibri">ELISA</font><font face="宋體">稀釋劑用于稀釋各種生物樣本，例如血液、血清和血漿。這可確保最佳濃度以有效結(jié)合和檢測(cè)目標(biāo)分析物，從而提供準(zhǔn)確可靠的檢測(cè)結(jié)果。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;">生物技術(shù)</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> 生物技術(shù)人員經(jīng)常使用</font><font face="Calibri">ELISA</font><font face="宋體">檢測(cè)進(jìn)行各種研究應(yīng)用，例如分析蛋白質(zhì)和抗體。這使得獲得準(zhǔn)確的結(jié)果變得至關(guān)重要，而無需反復(fù)執(zhí)行這些檢測(cè)。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> 這是通過使用</font><font face="Calibri">ELISA</font><font face="宋體">稀釋劑來稀釋樣品、降低背景噪音并最大限度地減少</font><font face="Calibri">ELISA</font><font face="宋體">反應(yīng)中不必要的相互作用來實(shí)現(xiàn)的。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><font face="宋體"> </font><font face="Calibri">ELISA </font><font face="宋體">稀釋劑，包括樣品稀釋劑和測(cè)定</font><font face="Calibri">/</font><font face="宋體">抗體稀釋劑，對(duì)于獲得準(zhǔn)確可靠的 </font><font face="Calibri">ELISA </font><font face="宋體">結(jié)果至關(guān)重要。樣品稀釋劑用于處理樣品，而測(cè)定</font><font face="Calibri">/</font><font face="宋體">抗體稀釋劑用于稀釋檢測(cè)抗體并優(yōu)化反應(yīng)環(huán)境。選擇合適的稀釋劑需要考慮多種因素，并仔細(xì)查閱供應(yīng)商提供的資料以及根據(jù)實(shí)驗(yàn)需求進(jìn)行驗(yàn)證。 試劑盒制造商的推薦通常是不錯(cuò)的選擇。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri; mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt; mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
找尋抗體，試劑，細(xì)胞裂解液，試劑盒
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