什么是ELISA微孔板?一文帶您了解類型、規(guī)格和應(yīng)用
<p> <font face="宋體" style="font-size: 10.5pt;"> 酶聯(lián)免疫吸附實驗(</font><span style="font-family: Calibri; font-size: 10.5pt;">ELISA</span><font face="宋體" style="font-size: 10.5pt;">)是一種常用的基于板的免疫學(xué)領(lǐng)域金標(biāo)準(zhǔn)測定法,用于生命科學(xué)和分子生物學(xué)實驗檢測和量化復(fù)雜混合物中的生物分子。</font></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 在技術(shù)上,將抗原或抗體包被在</font>96<font face="宋體">或</font><font face="Calibri">384ELISA</font><font face="宋體">微孔板的表面。然后,將微孔板與抗原特異性抗體一起孵育,這些抗體會與微孔板底部目標(biāo)抗原結(jié)合。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>ELISA<font face="宋體">板是</font><font face="Calibri">ELISA</font><font face="宋體">檢測的核心。它們也被稱為微量滴定板或微孔板,由聚苯乙烯或聚丙烯制成。如今,市場上有各種</font><font face="Calibri">ELISA</font><font face="宋體">板或微孔板可供選擇,以適應(yīng)不同的實驗室工作流程。因此,要開發(fā)精確、準(zhǔn)確和靈敏的生物測定,選擇合適的板至關(guān)重要,從而選擇合適的表面來固定目標(biāo)生物分子。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 在本文中,酶聯(lián)生物將為您介紹下</font>ELISA<font face="宋體">微孔板特點、市場上可買到的微孔板的格式及其多種應(yīng)用。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<h3><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>Elisa<font face="宋體">板類型</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></h3>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 微孔板有多種類型,每種類型都針對特定的檢測要求和檢測方法量身定制。每個微孔板的表面都對特定生物分子具有高結(jié)合親和力。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<ol>
<li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 親和免疫分析板</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 親和免疫測定板是為了增強抗原和抗體的結(jié)合而開發(fā)的。它為固定生物分子提供了高親和力的表面,從而提高了測定靈敏度、定量準(zhǔn)確度和最佳相互作用。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 這些板用于</font>ELISA<font face="宋體">和免疫測定。它們涂有鎳、鏈霉親和素、抗體和其他配體。它有多種類型,包括:</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 生物素結(jié)合板:包括</font>NeutrAvidin<font face="宋體">包被板和鏈霉親和素包被板。這些板通常用于以生物素化分子(如抗體或核酸)作為探針或檢測劑的檢測。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 抗體結(jié)合板:抗體結(jié)合板的類型包括蛋白</font>A/G/L<font face="宋體">包被板和二抗(</font><font face="Calibri">GAM</font><font face="宋體">、</font><font face="Calibri">GAR</font><font face="宋體">)包被板。它們與各種類型的免疫球蛋白(</font><font face="Calibri">IgG</font><font face="宋體">、</font><font face="Calibri">IgM</font><font face="宋體">和</font><font face="Calibri">IgA</font><font face="宋體">等)和一抗結(jié)合,用于高效抗原檢測。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>His<font face="宋體">標(biāo)簽結(jié)合板:鎳和銅涂層板是</font><font face="Calibri">His</font><font face="宋體">標(biāo)簽結(jié)合板的幾種類型。它們廣泛用于涉及組氨酸標(biāo)記蛋白的測定。</font><font face="Calibri">His</font><font face="宋體">標(biāo)簽結(jié)合板可實現(xiàn)</font><font face="Calibri">His</font><font face="宋體">標(biāo)簽蛋白的特異性固定和檢測。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>GST<font face="宋體">結(jié)合板:包括谷胱甘肽</font><font face="Calibri">(GSH)</font><font face="宋體">涂層板,用于檢測和分析</font><font face="Calibri">GST</font><font face="宋體">標(biāo)記或谷胱甘肽</font><font face="Calibri">S-</font><font face="宋體">轉(zhuǎn)移酶</font><font face="Calibri">(GST)</font><font face="宋體">融合蛋白。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
</ol>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font><strong><font face="宋體">吸收免疫分析板</font></strong></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 吸收免疫測定板也稱為結(jié)合免疫測定板。這些板是為蛋白質(zhì)、抗體和其他生物分子的被動吸附(包被)而開發(fā)的。它們被設(shè)計為專門針對特點的抗原。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 它也有兩種類型:</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<ol>
<li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 親水性蛋白質(zhì)結(jié)合板:它們旨在吸收親水性的生物分子,例如糖蛋白、抗體、聚糖和其他可溶性蛋白質(zhì)。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
<li><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 疏水性脂質(zhì)結(jié)合板:它們被設(shè)計用于吸收疏水性的生物分子,例如脂質(zhì)和脂蛋白。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li>
</ol>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> <strong>共價免疫分析板</strong></font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 這些專門設(shè)計的微孔板可實現(xiàn)肽、核酸和其他生物分子的共價連接。它們能夠與各種功能基團結(jié)合,包括氨基</font>(NH2)<font face="宋體">、巰基</font><font face="Calibri">(SH)</font><font face="宋體">、羧基</font><font face="Calibri">(COOH)</font><font face="宋體">和磷酸基</font><font face="Calibri">(PO4)</font><font face="宋體">,從而實現(xiàn)穩(wěn)定而高效的固定。它們可用于</font><font face="Calibri">ELISA</font><font face="宋體">和其他基于板的檢測技術(shù)。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 共價免疫測定板的一個例子是核酸測定板。它們有多種類型,以適應(yīng)寡核苷酸與各種功能基團或標(biāo)簽的結(jié)合。這些板可以通過共價鍵或親和相互作用實現(xiàn)牢固連接,確保在實驗中準(zhǔn)確可靠地分析核酸。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<h3><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>Elisa<font face="宋體">板工作原理</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></h3>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>ELISA<font face="宋體">板是免疫學(xué)和診斷實驗室中定期進行</font><font face="Calibri">ELISA</font><font face="宋體">檢測的必備材料。它確保目標(biāo)抗原的結(jié)合并保持目標(biāo)分析物和抗體之間的化學(xué)反應(yīng)。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 為了開發(fā)有效的免疫測定法,用適當(dāng)?shù)牡鞍踪|(zhì)或抗體包被</font>ELISA<font face="宋體">板至關(guān)重要。根據(jù)所需的檢測信號選擇合適的微孔板類型對于實現(xiàn)最佳的板包被至關(guān)重要。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 檢測微孔板顏色的選擇取決于所檢測的信號。例如,黑色或白色不透明板用于檢測化學(xué)發(fā)光和熒光信號,而透明聚苯乙烯平底板用于檢測比色信號。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 確保板上沒有劃痕,以免干擾分析和最終結(jié)果。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 包被過程涉及被動吸附,即將蛋白質(zhì)或抗體添加到板中,并讓其與塑料表面結(jié)合。通常,先制備蛋白質(zhì)或抗體溶液,然后在板上孵育特定時間。孵育后,除去包被溶液,并添加封閉緩沖液以防止非特異性結(jié)合。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 需要注意的是,理想包被條件和結(jié)合能力可能因所用的特定蛋白質(zhì)或抗體而異。通常需要通過實驗來確定適當(dāng)?shù)陌粷舛?。包被板的蛋白質(zhì)含量略高于所需量可實現(xiàn)更廣泛的檢測范圍,但包被量過大可能導(dǎo)致非特異性結(jié)合和高背景信號。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 板子包被后,可立即使用或儲存在</font>4°C<font face="宋體">以備將來使用,具體取決于包被蛋白的穩(wěn)定性。適當(dāng)?shù)膬Υ鏃l件可確保檢測結(jié)果的可靠性和可重復(fù)性。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<h3><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>ELISA<font face="宋體">板用途</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></h3>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>ELISA<font face="宋體">聚苯乙烯微孔板在研究、臨床和診斷實驗室及行業(yè)中有著廣泛的應(yīng)用。它使科研人員能夠量化樣本中目標(biāo)分子的存在和濃度,例如血液、血清、血漿、尿液或細胞裂解物。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 這些板具有多個孔或隔間,可同時評估多個樣品或測試條件。它們可進行高通量篩選,使研究人員能夠高效處理多個樣品。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>ELISA<font face="宋體">板兼容各種檢測方法,包括比色、熒光和化學(xué)發(fā)光技術(shù),為檢測和測量感興趣的抗原提供了靈活性。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font><strong>ELISA<font face="宋體">檢測</font></strong></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> </font>ELISA<font face="宋體">是實驗室中最常見和最廣泛使用的基于板的免疫測定技術(shù)之一。除了微量離心管和移液器外,該測定還需要</font><font face="Calibri">8</font><font face="宋體">孔條、</font><font face="Calibri">96</font><font face="宋體">孔或</font><font face="Calibri">384</font><font face="宋體">孔</font><font face="Calibri">ELISA</font><font face="宋體">中結(jié)合或高結(jié)合板。它們允許同時評估多種生物分子。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 對于從事組織培養(yǎng)或基于細胞的分析的研究人員來說,介質(zhì)結(jié)合</font>ELISA<font face="宋體">板為細胞粘附和生長提供了最佳表面。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 孵育和清洗培養(yǎng)板時,必須正確密封培養(yǎng)板,以防止交叉污染并保持樣品的完整性。培養(yǎng)板密封器可以簡化此過程。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 使用</font>ELISA<font face="宋體">板時,請考慮用于產(chǎn)生信號的底物。不同的底物提供各種檢測選項,包括比色和化學(xué)發(fā)光信號,讓您可以根據(jù)實驗需要選擇最合適的方法。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"> </span></p>
<p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><font face="宋體"> 以上就是關(guān)于</font>ELISA<font face="宋體">微孔板的介紹。選擇合適的微孔板對</font><font face="Calibri">ELISA</font><font face="宋體">實驗結(jié)果至關(guān)重要,建議根據(jù)實驗需求,例如結(jié)合類型和材質(zhì),仔細選擇合適的</font><font face="Calibri">ELISA</font><font face="宋體">微孔板,以確保實驗的成功。</font></span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:宋體; mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>